Constitutive expression of pathogenesis-related proteins PR-1, GRP, and PR-S in tobacco has no effect on virus infection.
نویسندگان
چکیده
منابع مشابه
Constitutive expression of pathogenesis-related proteins PR-1, GRP, and PR-S in tobacco has no effect on virus infection.
Samsun NN tobacco cells were transformed with chimeric genes for pathogenesis-related (PR) proteins derived from genomic (PR-1a, GRP) or cDNA (PR-S) clones under the transcriptional control of the cauliflower mosaic virus 35S promoter. Regenerated plants were assayed by RNA and protein gel blotting, and plants showing high specific expression of the inserted genes were selected for self-pollina...
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Type 1 allergies are recognized as an important disease affecting around 25% of the population of industrialized countries of the Northern hemisphere. Allergic patients produce specific IgE antibodies after frequent exposure to either inhaled or nutritive allergens. Of the plant allergens listed in the Official Allergen Database of the International Union of Immunological Societies, approx. 25%...
متن کاملStructure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group.
Infection of Samsun NN tobacco with tobacco mosaic virus (TMV) was found to induce the synthesis of mRNA encoding a basic protein with a 67% amino acid sequence homology to the known acidic pathogenesis-related (PR) proteins 1a, 1b and 1c. By Southern blot hybridization it was shown that the tobacco genome contains at least eight genes for acidic PR-1 proteins and a similar number of genes enco...
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The effect of ethephon (2-chloroetylphosphonic acid) on the degradation of proteins and on the induction of Lycopersicon esculentum pathogenesis-related (PR) proteins was studied in tomato leaf discs. The rate of ribulose, -1,5-bisphosphate carboxylase/oxygenase (Rubisco) degradation was maximal in discs after 48 hours of incubation with 1 millimolar ethephon, leading to complete disappearance ...
متن کاملThe nucleotide sequence of pathogenesis-related (PR) 1b protein gene of tobacco
A genomic clone encoding the pathogenesis-related (PR) lb protein was cloned from Nicotiana tabacum cv. Samsun NN genomic library screening with cDNA clone of PRla protein gene (1). The following nucleotide sequence contains coding and its flanking regions. The nucleotide sequence of PRlb protein gene is highly similar to that of PRla (2-5). Coding region is from 457 to 963 without intron and c...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 1989
ISSN: 1040-4651,1532-298X
DOI: 10.1105/tpc.1.3.285